Callus transformation
WebApr 7, 2024 · Crocus sativus is a recalcitrant plant for genetic transformation and genetic improvement, largely due to difficulties in Agrobacterium mediated transformation and … WebModification of the rice genome by Agrobacterium-mediated transformation is a general technique that can be easily performed today. Successful methods were established by vigorous studies on the culture system and the elucidation of Agrobacterium transformation mechanisms. This section provides a detailed description of routine and efficient ...
Callus transformation
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WebFeb 2, 2024 · Crop-improvement efforts for Chakhao black rice (Oryza sativa L.) through genetic engineering have not made much progress due to the absence of a protocol for callus induction and regeneration. We developed a tissue culture protocol for the Agrobacterium-mediated transformation of Chakhao Amubi and Chakhao Poireiton … WebNov 17, 2024 · The optimized parameters obtained in “Vector construction and transformation” were used for callus transformation. The GUS-positive callus was …
WebJun 25, 2024 · Embryogenic callus induction and Agrobacterium-mediated genetic transformation of 'Shine Muscat' grape Embryogenic callus induction and Agrobacterium -mediated genetic transformation of 'Shine Muscat' grape Plant Biotechnol (Tokyo). 2024 Jun 25;37 (2):185-194. doi: 10.5511/plantbiotechnology.20.0527a. Authors WebApr 1, 2024 · A higher callus induction rate from germinated seeds was obtained in N6 than in MS or B5 medium. Then the optimal transformation efficiency of the gus reporter gene was obtained by infection with Agrobacterium tumefaciens culture of OD 600 = 0.5 for 30 min, followed by 3 days of co-cultivation with 300 μmol/L acetosyringone.
WebJul 30, 2010 · The transformation experiment was performed using 4-week-old callus, which was previously cultured on the best callus maintenance medium as described above. Callus pieces (~250 mg in weight) were immersed in the Agrobacterium suspension for 30 min. The infected calli were dried on sterile filter paper to remove excess bacteria. WebJun 25, 2024 · We established a method for embryogenic callus induction and highly efficient Agrobacterium-mediated genetic transformation of a table grape cultivar 'Shine …
WebDec 1, 2024 · The transformation of callus tissue is standard practice for gene functional studies in citrus, as well as in other perennial woody plants. Here, the pFGC19 plasmid was optimised (Nelson et al. 2007 ) that carry different fluorescent protein tags and antibiotic selections, which could be used to generate transgenic lines expressing two ...
WebSep 27, 2013 · The callus genetic transformation with A. tumefaciens also promotend the gus gene transient expression on the callus co-cultiveted for 15 e 30 minutes. The transformed callus was transfered to a ... summer in the city steinbachWebVarious factor influencing transformation of rice callus and multiple shoots were optimized such as co-cultivation period (3 days) and bacterial inoculum density (0.6-0.8 at 600 nm ... summer in the city tabWebA callus consists of an amorphous mass of loosely arranged thin-walled parenchymatous cells arising from the proliferating cells of the parent tissue. More often callus is … summer in the city jordanhillWebApr 10, 2024 · Sugarcane is a complex polyploid aneuploid cash crop, and transgenic varieties are important for molecular genetic and traditional breeding approaches. Herein, the sugarcane variety ROC22 served as the receptor, the Bar gene served as a screening marker, and positive and negative fragments of the ScD27.2 gene, upstream of … summer in the city spoonfulWebSep 1, 2024 · The results indicated that the callus transformation system is efficient and adaptable for gene functional investigation in soybean genotype-, vector-, and … palanpur india shower facilitiesWebDec 30, 2011 · Steps for Agrobacterium mediated transformation and regeneration of rice calli.Four indica rice cultivars i.e. IR64, PB1, CSR10 and Swarna were used here. Steps a-f have to be performed under dark conditions. (a) Callus initiation on callus induction … summer in the city steinbach 2022WebMar 1, 2006 · The transformation efficiency exceeded 40% of the explants. This protocol could become a powerful tool for functional genomics in tomato. ... The explants were then subcultured in callus induction medium containing 100 mg l –1 kanamycin, 1.5 mg l –1 zeatin and 375 mg l –1 Augmentin (GlaxoSmithKline, Uxbridge, UK). Augmentin, ... summer in the city lovin spoonful chords