WebDec 31, 2024 · A species-specific chloroplast vector for Capsicum annuum L was designed to target homologous regions trnA and trnI of IR region and its transformation efficiency compared to a heterologous vector was found to be 20%, compared to 2%, found with two plastid vectors. Expand 5 PDF View 5 excerpts, references background and … Chloroplast transformation involves modification of the genome or introduction of a new foreign gene into the chloroplast. This process involves the designing of the DNA construct, including the selectable markers, as well as the introduction of the DNA construct to the chloroplast. Design of vector and selectable … See more Before the DNA construct or also known as a vector is introduced to the chloroplast, the DNA construct will need to undergo modification … See more Once the vector for the chloroplast has been successfully constructed, it is introduced into the chloroplast for the transformation process. The transformation method indicates the introduction of … See more
Expression of β-carotene ketolase gene from the green alga ...
WebOttelia cordata is a typical submerged plant to the family of hydrocharitaceae. In this work, complete chloroplast genome of O. cordata was assembled and characterized from high-throughput Illumina s WebConstruction of chloroplast transformation vector and its functional evaluation in Momordica charantia L 3 Biotech. 2024 Mar;8 (3):140. doi: 10.1007/s13205-018-1160-z. Epub 2024 Feb 19. Authors Muralikrishna Narra 1 , Srinivas Kota 1 , Yashodhara Velivela 1 , Raghu Ellendula 1 , V Rao Allini 1 , Sadanandam Abbagani 1 Affiliation iowa and trump
A Modular Cloning Toolbox for the Generation of Chloroplast
WebFeb 3, 2010 · Therefore, chloroplast transformation vectors were made for tobacco ( Nicotiana tabacum) and lettuce ( Lactuca sativa ), with endogenous (Nt-Nt, Ls-Ls) or heterologous (Nt-Ls, Ls-Nt) psbA promoter, 5′ UTR and 3′ UTR, regulating expression of the anthrax protective antigen (PA) or human proinsulin (Pins) fused with the cholera toxin B … WebDec 6, 2007 · Chloroplast vector systems used in these biotechnology applications are described. ADVANTAGES OF PLASTID TRANSFORMATION. Chloroplasts are … WebMay 1, 2002 · The resulting 1,826-bp PCR product was ligated into a TA cloning vector (pCR1000, Invitrogen, Carlsbad, CA) to give pSMR1000. The chloroplast genomes of wild-type and transformed cells were analyzed with primer pairs derived from the vector and AHAS gene sequences. iowa and richmond score