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Chloroplast transformation vector

WebDec 31, 2024 · A species-specific chloroplast vector for Capsicum annuum L was designed to target homologous regions trnA and trnI of IR region and its transformation efficiency compared to a heterologous vector was found to be 20%, compared to 2%, found with two plastid vectors. Expand 5 PDF View 5 excerpts, references background and … Chloroplast transformation involves modification of the genome or introduction of a new foreign gene into the chloroplast. This process involves the designing of the DNA construct, including the selectable markers, as well as the introduction of the DNA construct to the chloroplast. Design of vector and selectable … See more Before the DNA construct or also known as a vector is introduced to the chloroplast, the DNA construct will need to undergo modification … See more Once the vector for the chloroplast has been successfully constructed, it is introduced into the chloroplast for the transformation process. The transformation method indicates the introduction of … See more

Expression of β-carotene ketolase gene from the green alga ...

WebOttelia cordata is a typical submerged plant to the family of hydrocharitaceae. In this work, complete chloroplast genome of O. cordata was assembled and characterized from high-throughput Illumina s WebConstruction of chloroplast transformation vector and its functional evaluation in Momordica charantia L 3 Biotech. 2024 Mar;8 (3):140. doi: 10.1007/s13205-018-1160-z. Epub 2024 Feb 19. Authors Muralikrishna Narra 1 , Srinivas Kota 1 , Yashodhara Velivela 1 , Raghu Ellendula 1 , V Rao Allini 1 , Sadanandam Abbagani 1 Affiliation iowa and trump https://hypnauticyacht.com

A Modular Cloning Toolbox for the Generation of Chloroplast

WebFeb 3, 2010 · Therefore, chloroplast transformation vectors were made for tobacco ( Nicotiana tabacum) and lettuce ( Lactuca sativa ), with endogenous (Nt-Nt, Ls-Ls) or heterologous (Nt-Ls, Ls-Nt) psbA promoter, 5′ UTR and 3′ UTR, regulating expression of the anthrax protective antigen (PA) or human proinsulin (Pins) fused with the cholera toxin B … WebDec 6, 2007 · Chloroplast vector systems used in these biotechnology applications are described. ADVANTAGES OF PLASTID TRANSFORMATION. Chloroplasts are … WebMay 1, 2002 · The resulting 1,826-bp PCR product was ligated into a TA cloning vector (pCR1000, Invitrogen, Carlsbad, CA) to give pSMR1000. The chloroplast genomes of wild-type and transformed cells were analyzed with primer pairs derived from the vector and AHAS gene sequences. iowa and richmond score

A Modular Cloning Toolbox for the Generation of Chloroplast

Category:Improved in planta genetic transformation efficiency in bitter …

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Chloroplast transformation vector

Role of Heterologous Chloroplast Sequence Elements in …

WebThe chloroplast genome can be transformed through homologous recombination, wherein the transformation vector containing the gene of interest (flanked between two host DNA segments) is... WebConstruction of chloroplast transformation vector and its functional evaluation in Momordica charantia L 3 Biotech. 2024 Mar;8 (3):140. doi: 10.1007/s13205-018-1160-z. …

Chloroplast transformation vector

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WebThe vector for chloroplast transformation is based on the selectable marker gene aadA that provides resistance to antibiotic spectinomycin. The single foreign (desirable) gene … WebJun 14, 2007 · A universal transformation vector (pASCC201) for Brassica chloroplast was constructed with trnV – rrn16S (left) and trnI – trnA – rrn23S (right) of the IR A region as a recombination site for the transformed gene. In transforming plasmid pASCC201, a chimeric aadA gene was cloned between the rrn16S and rrn23S plastid gene borders.

WebThe vector p64Dbkt was transferred to the chloroplast genome of C. reinhardtii using a micro-particle bombardment. The transgenic alga was selected and maintained in the TAP agar plates containing 100 mg/L spectinomycin. PCR and Southern blot analyses indicated that bkt was integrated into the chloroplast genome of the transformants.

WebJun 21, 2024 · Chloroplasts are a particularly attractive subcellular compartment for foreign gene expression 7, 8, 9 because of (1) the possibility of achievement of high-yield protein … WebJul 23, 2011 · Chloroplast transformation of alfalfa ( Medicago sativa L.), a useful forage plant with high market value, was achieved using a vector carrying aadA and gfp genes being introduced into the chloroplasts of alfalfa via particle bombardment using leaves and calli as explants.

WebThe following points highlight the top five methods of chloroplast transformation in higher plants. The methods are: 1. Vectors for Plastid Transformation 2. Engineering for High …

WebSep 23, 2024 · Structure of Chloroplasts. Chloroplasts can be found in the cells of the mesophyll in plant leaves. There are usually 30-40 per mesophyll cell. The chloroplast has an inner and outer membrane with ... iowa and sdsuWebFeb 11, 2024 · The transformation of chloroplast or plastid genomes in higher plants represents a promising technology in multiple biotechnological applications such as the introduction of agronomically important traits, metabolic engineering, recombinant protein expression and the production of high-value therapeutic compounds (Maliga and Bock, … onyx creative tucson azWebSep 2, 2024 · The chloroplast transformation vectors pSyHPT (single-gene construct) and pTop2 (operon construct) were designed to carry out the transformation. The polycistronic construct (pTop2) was designed by spacing the genes with two identical intercistronic expression elements (IEE). onyx creative wandWebApr 3, 2008 · Chloroplast targeting vector, containing flanking sequences specific to the genome to be targeted ( Fig. 2) Chloroplast expression cassette, containing the … onyx creative addressWebA chloroplast (/ ˈ k l ɔːr ə ˌ p l æ s t,-p l ɑː s t /) is a type of membrane-bound organelle known as a plastid that conducts photosynthesis mostly in plant and algal cells.The photosynthetic pigment chlorophyll captures the … onyx crystal ballWebPlastids (chloroplasts) are the defining organelles of plants and eukaryotic algae. In addition to performing photosynthesis, plastids harbor numerous other metabolic pathways and therefore are often referred to as the … iowa and texasWebOct 10, 2014 · In a three-level assembly process, we established a vector fostering gene expression and formation of griffithsin, a potential viral entry inhibitor and HIV … onyx creative architects