WebbpUO-SeVR1 is a resistance derivative of pSEV, the virulence plasmid specific of Salmonella enterica serovar Enteritidis. It was first detected in a Spanish isolate involved in gastroenteritis, but closely related plasmids are widespread in highly invasive isolates originating from Africa.According to its nucleotide sequence, pUO-SeVR1 consists of … WebbPurpose: The plasmid-borne mobilized colistin resistance gene ( mcr-1) was discovered in 2015. Subsequently, the rapid horizontal transfer of mcr-1 gene to diverse bacterial species poses a serious threat to public health, which urgently needs the introduction of novel antimicrobial strategies.
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Webb5 juni 2015 · Finally, bacteria were grown on chloramphenicol and 1% arabinose LB-plates at 32 °C for 1 to 2 days and kanamycin-sensitive colonies were identified by replica plating. BAC DNA was isolated from bacteria using the BAC preparation protocol of the PureLink HiPure Plasmid Maxiprep kit (Invitrogen, Karlsruhe, Germany). WebbAdvantages of the PureLink™ HiPure Plasmid Maxiprep Kit include: • Labor and time saving—no need for extra steps to remove endotoxin and other contaminants • High … lambeth additional hmo
PureLink™ HiPure Expi Plasmid Megaprep Kit - Thermo Fisher …
WebbUse 100 mL (high copy number plasmid) or 250–500 mL (low copy number plasmid) of an overnight culture. Note • Use the PureLink™ HiPure Precipitator Module (Cat. no. … Webb18 jan. 2014 · 产品说明 本试剂盒采用离心柱特异吸附DNA，适合于从1-4 ml培养的细菌细胞中高效地提取质粒DNA。 溶液包含指示剂，通过颜色的变化，指示裂解、中和是否完全，从而保证质粒提取的质量，实现操作的可视化。 用本试剂盒提取得到的质粒DNA可用于分子生物学各种常规实验，包括酶切、连接、转化、测序等多种操作。 特点： (1) 溶 … WebbPureLink™ HiPure Plasmid Filter Purification Kits - for Midi and Maxi preparation of Plasmid DNA Qualitative Verification of Target Sequence Using Platinum® Taq DNA Polymerase SILANE Genomic DNA ssM13 DNA Isolation TRI Reagent® DNA/Protein Isolation Protocol mRNA Protocols › RNA Protocols › lambeth active