Hmtiia
Websequence of the hMTIIA gene by mobility shift assay. A short oligonucleotide containing the MREa sequence was used as a probe (Figs. 1, A and B), and a crude extract of HeLa cells was used as the ... WebApr 19, 2000 · Metallothionein overexpression in these cells was not associated with an increased copy number of the hMTIIa gene or with different transfection efficiencies. Furthermore, we showed by reverse transcription-polymerase chain reaction analysis that transfection of the pMT-TK plasmid results in a 56-fold higher expression of thymidine …
Hmtiia
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WebIn humans, the predominant metallothionein gene is hMTIIA, which accounts for 50% of all metallothioneins expressed in cultured human cells. The hMTIIA promoter is quite … WebAcronym. Definition. HCIA. Hyper Converged Infrastructure Appliance. HCIA. Hudson County Improvement Authority (New Jersey) HCIA. Hawaii Crop Improvement …
WebIn humans, the predominant metallothionein gene is hMTIIA, which accounts for 50% of all metallothioneins expressed in cultured human cells. The hMTIIA promoter is quite complex. WebThe CAT-Tox (L) assay has recently been developed and validated for detecting and quantifying the specific molecular mechanisms that underlie toxicity of various xenobotic chemicals. We performed this assay to measure the transcriptional responses associated with 2,4,6-trinitrotoluene (TNT) and 2 of …
WebApr 14, 2024 · The rWNiCo mixture (5-50 microg/ml) showed dose-related induction of the GSTYA, hMTIIA, p53RE, FOS, NFkappaBRE, HSP70, and CRE promoters. An examination of the pure metals, tungsten (W), nickel (Ni), and cobalt (Co), comprising the rWNiCo mixture, demonstrated that each metal exhibited a similar pattern of gene induction, but …
WebHuman metallothionein genes--primary structure of the metallothionein-II gene and a related processed gene. Nature 299: 797-802, 1982. PubMed: 7133118. Hyland VJ, et al. …
WebThe gene hMTIIA produces a protein that binds to heavy metals and protects cells from their toxicity. The gene is transcribed at low levels in all cells but is induced to express at high levels in cells exposed to heavy metals. To use RNAi (RNA interference) as a research tool, investigators introduce short synthetic _____ RNA into cells. ... order of 09/11/1994WebApr 19, 2000 · Because the hMTIIa promoter limits expression to cisplatin-resistant cells, delivery systems other than direct injection of the plasmid into the tumor could be utilized. For example, intraperitoneal administration could be used to treat cisplatin-resistant ovarian peritoneal carcinomatosis. Intraperitoneal administration might enable expression ... order of 007 movies with daniel craighttp://genesdev.cshlp.org/content/5/4/670.full.pdf how to transfer contacts to jitterbug phoneWeb1A1 (CYP1A1), metallothionein (HMTIIA), retinoic acid response element (RARE) and 53-kDa tumor suppressor protein response element (p53RE). Figures 2 and 3 present the fold inductions of the Ya subunit of the glutathion s-transferase (GSTYa) gene promoter and the xenobiotic response element (XRE) in HepG2 cells exposed to iprodione. Data ... how to transfer contacts to sd card androidWebSep 29, 1997 · The NF1-A repression domain inhibited the promoter activities of not only the hMTIIA gene but also those of the GST-P and CCAAT/enhancer binding protein delta genes. RD1 and RD2 had abundant serine and glycine residues, and proline and serine residues, respectively. Whereas some repression domains identified previously are … order of 1616WebTo test this hypothesis, we performed the MTT-assay for cell viability, the CAT-Tox (L) assay for gene induction, and the Western Blot analysis to assess the expression of cellular proteins including c-fos, HMTIIA, HSP70 and p53. Data obtained from the MTT assay indicated a strong dose-response relationship with respect to arsenic trioxide ... how to transfer corporate stock certificatesWebAug 30, 1996 · A partially purified protein fraction containing replication protein A (RPA) binds to the hMTIIA gene transcription start site and represses transcription from the hMTIIA promoter in vitro. In addition, overexpression of the human 70-kDa RPA-1 protein represses transcription of a reporter gene controlled by the hMTIIA promoter in vivo. how to transfer contacts on flip phones