Webbmethod, injectoporation is just at the beginning of its evaluation by different laboratories [ 1 ] and appears to be promising. The technique of intrauterine electroporation is ef cient … Webb13 maj 2024 · Injectoporation of Ripor2-constructs, immunostaining and antibodies For injectoporation, the organ of Corti was isolated and placed in DMEM/F12 medium with 1.5 µg/ml ampicillin. Glass electrodes (~2 µm diameter) were used to deliver the plasmid (500 ng/µl in Hank’s Balanced Salt Solution (HBSS)) to the sensory epithelium.
[PDF] Using injectoporation to deliver genes to mechanosensory …
Webb29 mars 2024 · Culture and injectoporation of cochlear explants. Injectoporation of cochlear explants was performed following recently established protocols (Xiong et al., 2014). In brief, cochleae were dissected from postnatal (P) day 4 and P5 wild-type mice, cut into three pieces, and cultured for 4 h in DMEM/F12 medium with 10 ng/μl ampicillin. WebbInjectoporation allows for gene transfer into dozens of hair cells, and it is compatible with the analysis of hair cell function using imaging approaches and electrophysiology. Tissue dissection and injectoporation can be carried out within a few hours, and the tissue can be cultured for days for subsequent functional analyses. gregg hurwitz tim rackley series
Using injectoporation to deliver genes to mechanosensory hair …
WebbJohns Hopkins University School of Medicine. The Solomon H. Snyder Department of Neuroscience. Baltimore, MD 21205 WebbTo investigate whether GRXCR2 (Δ36–95) is also concentrated at the base of stereocilia, injectoporation (Xiong et al., 2014) was performed to express Myc-tagged GRXCR2 (Δ36–95) in P3 hair cells. Two days after injectoporation, hair cells were fixed and Myc-antibody was used to detect Myc-GRXCR2 (Δ36–95). Webb30 okt. 2024 · Injectoporation of untagged taperin also led to the extraordinary growth of stereocilia, excluding the possibility that the over-elongation of stereocilia was caused by Myc-tag-induced aberrant protein activity (Figure S4A). The 30 aa from 90–121 in taperin are important for the interaction with GRXCR2 (Figure 2A). gregg hyatt american family ins