WebMetachromasia may be defined as the staining of tissue or tissue components such that the color of the tissue-bound dye complex differs significantly from the color of the original … WebTissue components often demonstrated by metachromatic stains : Anyloid material, Mast cell granules Mucin Cartilage Direct staining Application of simple dye to stain the tissue in varying shades of colours. Indirect staining It means use of mordant of facilitate a particular staining method or the use
12 METACHROMATIC STAINING - National Institute of Open …
Metachromasia (var. metachromasy) is a characteristical change in the color of staining carried out in biological tissues, exhibited by certain dyes when they bind to particular substances present in these tissues, called chromotropes. For example, toluidine blue becomes dark blue (with a colour range from blue-red dependent on glycosaminoglycan content) when bound to cartilage. Other widely used metachromatic stains are the haematological Giemsa and May-Grunwald stains tha… Webcellular structures stain orthochromatically with internal dye concentrations of such metachromatic dyes as methylene blue and toluidine blue which, if in solu- tion, would be metachromatic. Therefore, at ordinary levels of staining these sub- stances depress the natural tendency of these dyes to change color. bismuth 4제요법
Chemical Basis for the Histological Use of Safranin O in the
WebBetter staining of nuclei and connective tissue; Cytoplasmic staining –enhanced with acidic dyes. Nuclear chromatin shown in detail; Preservation of details for photography. Best results with metachromatic stain; B5 fixative i s frequently used for b one marrow,spleen, lymph nodes and other hematopoetic tissue; Disadvantages of Hg fixatives: WebThe influence of different histochemical conditions on some metachromatic staining reactions has been studied using polyacrylamide films containing pure … WebMaybe tested with 10cc 37-40% formaldehyde o Rapid reddish-purple discoloration means it is still good o If the reaction is delayed and the color is deep blue-purple then the soln is breaking down. GLYCOGEN. ... Acid mucopolysaccharides o Metachromatic stains Toluidine blue and Azure A Uranyl nitrate Azure method o Alcian blue technique; bismuth 524